This Mouse Fatty Acid Binding Protein Intestinal (FABP2) ELISA Kit from Innovative Research is intended for Quantitative Detection of mouse FABP2 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse FABP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FABP2 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A2-E132) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FABP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FABP2 amount of sample captured in plate.
- Detection Target: Fatty Acid Binding Protein Intestinal (FABP2)
- Uniprot ID: )
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 31.2pg/ml-2000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.; Immunogen sequence: 333
